Three-dimensional co-culture of mesenchymal stromal cells and differentiated osteoblasts on human bio-derived bone scaffolds supports active multi-lineage hematopoiesis in vitro: Functional implication of the biomimetic HSC niche.

Recent studies have indicated that the hematopoietic stem/progenitor cell (HSPC) niche, consisting of two major crucial components, namely osteoblasts (OBs) and mesenchymal stromal cells (MSCs), is responsible for the fate of HSPCs. Thus, closely mimicking the HSPC niche ex vivo may be an efficient strategy with which to develop new culture strategies to specifically regulate the balance between HSPC self-renewal and proliferation. The aim of this study was to establish a novel HSPC three-dimensional culture system by co-culturing bone marrow-derived MSCs and OBs differentiated from MSCs without any cytokines as feeder cells and applying bio-derived bone from human femoral metaphyseal portion as the scaffold. Scanning electron microscopy revealed the excellent biocompatibility of bio-derived bone with bone marrow-derived MSCs and OBs differentiated from MSCs. Western blot analysis revealed that many cytokines, which play key roles in HSPC regulation, were comprehensively secreted, while ELISA revealed that extracellular matrix molecules were also highly expressed. Hoechst 33342/propidium iodide fluorescence staining proved that our system could be used to supply a long-term culture of HSPCs. Flow cytometric analysis and qPCR of p21 expression demonstrated that our system significantly promoted the self-renewal and ex vivo expansion of HSPCs. Colony-forming unit (CFU) and long-term culture-initiating cell (LTC-IC) assays confirmed that our system has the ability for both the expansion of CD34+ hematopoietic stem cells (HPCs) and the maintenance of a primitive cell subpopulation of HSCs. The severe-combined immunodeficient mouse repopulating cell assay revealed the promoting effects of our system on the expansion of long-term primitive transplantable HSCs. In conclusion, our system may be a more comprehensive and balanced system which not only promotes the self-renewal and ex vivo expansion of HSPCs, but also maintains primitive HPCs with superior phenotypic and functional attributes

Can Distribution Grids Significantly Contribute to Transmission Grids' Voltage Management?

Power generation in Germany is currently transitioning from a system based on large, central, thermal power plants to one that heavily relies on small, decentral, mostly renewable power generators. This development poses the question how transmission grids' reactive power demand for voltage management, covered by central power plants today, can be supplied in the future. In this work, we estimate the future technical potential of such an approach for the whole of Germany. For a 100% renewable electricity scenario we set the possible reactive power supply in comparison with the reactive power requirements that are needed to realize the simulated future transmission grid power flows. Since an exact calculation of distribution grids' reactive power potential is difficult due to the unavailability of detailed grid models on such scale, we optimistically estimate the potential by assuming a scaled, averaged distribution grid model connected to each of the transmission grid nodes. We find that for all except a few transmission grid nodes, the required reactive power can be fully supplied from the modeled distribution grids. This implies that - even if our estimate is overly optimistic - distributed reactive power provisioning will be a technical solution for many future reactive power challenges

Study on Small Layers Producing Condition by Using the Method of Fuzzy Comprehensive Evaluation

Before the subdivision adjustment of single well layers carried out, we need to evaluate the producing degree of the existing layers. There are many factors affecting the properties of small layers. This paper, using fuzzy comprehensive evaluation method, calculates the comprehensive evaluation coefficient to judge the property of each small layer

A Bayesian Network Meta-Analysis Comparing the Efficacies of Eleven Novel Therapies with the Common Salvage Regimen for Relapsed or Refractory Acute Myeloid Leukemia

Background/Aims: Acute myeloid leukemia (AML) is a relapsed and refractory hematological malignancy with a lower morbidity but higher mortality. In addition to hematopoietic stem cell transplantation, chemotherapy is used as the front-line treatment. However, the diversity of available agents and the inconsistency of outcomes of relevant trials render treatment decision-making tough. Network meta-analysis (NMA) is an efficient statistical framework that makes a comprehensive comparison and provides a valuable clinical reference. Methods: All the potential trials were retrieved from the medical database and screened according to the inclusion and exclusion criteria. The main characteristics of each trial as well as the primary outcomes, including complete remission (CR), overall response rate (ORR), overall survival (OS), and event-free survival (EFS), were extracted. In addition, the network graph was plotted to illustrate the connections among the trials involved. Comparison results in the network were exhibited in a forest plot. Furthermore, the surface under the cumulative ranking curve (SUCRA) was introduced to rank the treatments for each endpoint. Results: A total of 11 trials were selected from 1,625 identifications. No significant difference in the common treatment was observed for the endpoints CR and ORR. In terms of OS, CPX-351 (HR: 0.77, 95% CrI: 0.63, 0.94) and HiDAC plus MK-8776 (HR: 0.80, 95% CrI: 0.68, 0.93) showed a superiority over the common salvage regimen in the short term, while HiDAC plus MK-8776 (HR: 0.80, 95% CrI: 0.70, 0.93) and Ara-C plus vosaroxin (HR: 0.86, 95% CrI: 0.74, 0.99) outperformed the common salvage regimen for the 3-year OS. In addition, clofarabine plus Ara-C (HR: 0.61, 95% CrI: 0.53, 0.69) and CPX-351 (HR: 0.71, 95% CrI: 0.60, 0.83) were confirmed to be efficacious in enhancing the rate of EFS. Conclusion: Referring to the network outcome and SUCRA value, clofarabine plus Ara-C (CR: 79.05%, ORR: 80.02%) and Ara-C plus vosaroxin (CR: 75.42%, ORR: 73.43%) were potentially the top two choices for both CR and ORR. CPX-351 (1-year OS: 91.36%), HiDAC plus MK-8776 (3-year OS: 94.23%) and clofarabine plus Ara-C (1-year EFS: 97.34%) yielded the highest probabilities to be the optimal choices for 1-year OS, 3-year OS and 1-year EFS, respectively

Analysis of thermal processing of liquid eggs using a high frequency ohmic heating: Experimental and computer simulation approaches

Experimental and simulated approaches combining an ohmic heating (OH) process and an external heating system for the pasteurization of liquid egg whites, yolks, and whole eggs were evaluated in this study.3D computer simulation models were developed for each approach using COMSOL Multiphysics 5.6 to visualize the temperature and distribution of degrees of pasteurization at several cross sections.From both the hot and cold spots of the samples, the temperature, thermal protein non-denaturation ratio (X), and pasteurization profiles were estimated.Color changes were also evaluated using a computer vision system (CVS). The heating conditions were optimized according to a high constant X value, a low total color difference value (ΔE < 3), and certain pasteurization standards for each sample.OH treatment combined with concurrent external heating approaches reduced the process time and prevented local overheating.These results are of potential value for the design of ohmic pasteurization systems for liquid eggs.公開日: 2022-08-1

Multi-Load Mode Analysis for Electric Vehicle Wireless Supply System

When electric vehicles (EVs) run on the wireless power supply coils, the multi-load working mode will appear, showing that that more than one EV is collecting energy from one coil. Aiming at the stability problem of multi-load mode, this paper mainly analyzes how the number of loads influences the system stability and defines the boundary condition of the load quantity. Meanwhile, an L-shaped coil structure and the T-shaped magnetic core structure are proposed to solve the problem of coil breakdown for high-power supply situations. The proposed structures effectively reduce the supply coils&rsquo; self-inductance on the premise of guaranteeing the power transfer, and ensure the security of the multi-load system. At last, the validity of theoretical analysis is verified by simulations and experiments

Chemicals Weaken Shoal Preference in the Rare MinnowGobiocypris rarus

Fish behavioral responses are sensitive to chemicals in the water. We tested rare minnow tested for their shoal preference, and the shoal (school) factors including nutritional status, body size, and shoal (school) size that can make their preference most stable were measured. Then shoal preference was measured again while fish and shoal were subjected to a concentration gradient of chemicals (cadmium ion [Cd2+], tricaine methanesulfonate [MS222], andp-chloroaniline). The results showed that single rare minnow preferred shoals over blank control tanks. In addition, this preference was most stable when the shoal was well fed and contained 20 individuals 2 cm long. Although there was no significant response after exposure top-chloroaniline, the time spent from entering the tank to start moving decreased greatly at concentrations of Cd2+>3 mg/L and MS222 >11 mg/L. The time the test fish spent close to the shoal significantly decreased at Cd2+>3 mg/L, MS222 >11 mg/L, andp-chloroaniline >10 mg/L, and the frequency of boundary line crossing increased at the same concentrations. The behavioral parameters changed by 20, 5, and 8 min once the lowest-observed-effect concentrations of Cd2+, MS222, andp-chloroaniline, respectively, were added. Our study provides useful information on rare minnow shoal preference that may be used for a biological early warning system.Environ Toxicol Chem2020;00:1-10. (c) 2020 SETA